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Scientific Experience

Manipulation of

Recombinant DNA

Basic technologies of recombinant DNA procedures, gene expression, isolation and identification of nucleic acids and proteins.

Microbial Metabolic Regulation 

An integrative perspective on bacterial physiology and metabolism through analysis of metabolic regulatory functions.

Protein Purification 

Lectures:

  • Introduction to Liquid Chromatography

  • Protein Purification Basics

  • Liquid Chromatography – Ion Exchange

  • Liquid Chromatography – Hydrophobic Interaction

  • Liquid Chromatography – Gel Filtration/Affinity

  • Protein Folding and Re-folding

  • Downstream Processing

Labs:

  • FPLC Operation – Purification of Protein Standards -1 week  

  • AEC purification of Thermotoga maritima -gal from Heat-Treated E. coli -1 week  

  • Purification of Thermotoga maritima -gal from E. coli by AEC, HIC, HAP -3 weeks

  • Purification of Thermotoga maritima -gal from E. coli by His-tag/IMEC -1 week  

  • Finish assays and run gels -1 week 

Comparative Plant Transcriptomics

  • Overview of Next Generation Sequencing technologies

  • How to design your RNA-seq experiments

  • Linux for biologists

  • Building a refernce transcriptome and aligning sequence data

  • Quantification and normallization of sequence reads

  • Differential Expression Analysis and GO Term enrichment

  • Comparing divergence in orthology, synteny, and expression patterns across plant species

Ethical Issues in Biotechnology

  • Explore fundamental ethics and responsible conduct of research.

  • Investigate current controversial issues in biotechnology.

  • Discuss new technologies in a rational and thoughtful way.

  • 90 minute seminar presentation 

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